Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody

Overview

Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody is an antibody targeting APOB. Common applications include WB, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: AOIH-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 21 kDa; calculated MW: 515605 MW.

Boster Bio Anti-ApoB/Apolipoprotein B Rabbit Monoclonal Antibody catalog # M00100. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: APOB — Apolipoprotein B-100
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: AOIH-1; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 21 kDa; Calculated: 515605 MW

Biological background

Protein function (datasheet): Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Cellular localization (datasheet): Cytoplasm . Secreted .

Tissue details (datasheet): Detected in astrocytoma, neuroblastoma and adrenal cortex cell lines. Some isoforms are detected in foreskin fibroblast cell lines, however isoform 17, isoform 18 and isoform 19 are not detected in these cells. .

Research relevance and current trends

• Commonly studied in contexts related to Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Cholesterol Metabolism,Heart Disease,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Lipids/Lipoproteins,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Pathways and Processes,Signal Transduction.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.