| Field | Specification |
|---|---|
| Target | |
| Alternative names | DNA dC->dU-editing enzyme APOBEC-3B; A3B; Phorbolin-1-related protein; Phorbolin-2/3; APOBEC3B |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human APOBEC3B. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets DNA dC->dU-editing enzyme APOBEC-3B (gene APOBEC3B) in human samples and is validated for Western blot. The predicted molecular weight is 45.9 kDa, and the supplier reports an observed band at about 35 kDa.
APOBEC3B is a nuclear cytidine deaminase of the APOBEC3 family that converts cytosine to uracil in single-stranded DNA. It restricts retroviruses and retrotransposons, and because its activity can mutate the host genome, it is widely studied as a driver of APOBEC-type mutation signatures in cancer.
| Target | DNA dC->dU-editing enzyme APOBEC-3B (gene APOBEC3B; UniProt Q9UH17, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 45.9 kDa |
| Observed MW | 35 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL/ml (Human) |
|---|
Samples with a confirmed band (WB): human HepG2 cells, human Jurkat cells, human THP-1 cells, human HEK293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the middle region of human APOBEC3B.
Reactivity Notes
The supplier lists reactivity with human. UniProt places DNA dC->dU-editing enzyme APOBEC-3B in the nucleus. Tissue expression noted by UniProt (human): Expressed at high and moderate levels in peripheral blood leukocytes, spleen, testes, heart, thymus, prostate and ovary. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
