| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein C-III; Apo-CIII; ApoC-III; Apolipoprotein C3; APOC3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human APOC3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17A47 is a rabbit monoclonal antibody that recognizes apolipoprotein C-III (gene APOC3) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 10.9 kDa, and the supplier reports an observed band at about 11 kDa.
Apolipoprotein C-III (APOC3) is a component of VLDL and HDL that raises plasma triglyceride levels. It inhibits lipoprotein lipase and slows the uptake of triglyceride-rich lipoprotein remnants by the liver, which has made it an important target in studies of triglyceride metabolism and cardiovascular risk.
| Target | Apolipoprotein C-III (gene APOC3; UniProt P02656, human) |
|---|---|
| Clone | 17A47 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 10.9 kDa |
| Observed MW | 11 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide based on the sequence of human APOC3.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein C-III as a secreted protein. Tissue expression noted by UniProt (human): Liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
