| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein D; Apo-D; ApoD |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ApoD |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 23A74 is a rabbit monoclonal antibody that recognizes apolipoprotein D (gene APOD) in human samples and is validated for Western blot and IHC. The predicted molecular weight is 21.3 kDa, and the supplier reports an observed band at about 28 kDa.
Apolipoprotein D (APOD) is a member of the lipocalin family that binds small hydrophobic molecules. It is found in plasma lipoproteins and is highly expressed in the nervous system, where it is studied in lipid transport, protection against oxidative stress and responses to nerve injury and aging.
| Target | Apolipoprotein D (gene APOD; UniProt P05090, human) |
|---|---|
| Clone | 23A74 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 21.3 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human plasma.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human apoD.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein D as a secreted protein. Tissue expression noted by UniProt (human): Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
