| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor-related protein 8; LRP-8; Apolipoprotein E receptor 2; LRP8; APOER2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ApoER2/LRP8 recombinant protein (Position: R444-D960). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects low-density lipoprotein receptor-related protein 8 (gene LRP8) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 105.6 kDa, and the supplier reports an observed band at about 106 kDa.
LRP8, also called ApoER2, is a member of the LDL receptor family that binds Reelin and apolipoprotein E-containing ligands. Together with the VLDL receptor it relays Reelin signals to the adaptor DAB1, guiding neuronal layering in the developing forebrain, and it also contributes to sperm maturation in the epididymis and to selenium uptake by selenoprotein P.
| Target | Low-density lipoprotein receptor-related protein 8 (gene LRP8; UniProt Q14114, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.01 mg NaN3 |
| Calculated MW | 105.6 kDa |
| Observed MW | 106 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 4 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human placenta tissue, human U87 cells, human A549 cells, human U937 cells, human HL-60 cells, human A431 cells, human HeLa cells, rat testis tissue, rat thymus tissue, rat spleen tissue, rat heart tissue, mouse testis tissue, mouse thymus tissue, mouse heart tissue, mouse RAW264.7 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue, human mammary cancer tissue, human rectal cancer tissue, mouse intestine tissue, rat intestine tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with a confirmed signal (flow cytometry): U87 cells, HL-60 cells.
Immunogen
Recombinant human ApoER2/LRP8 fragment (Arg444–Asp960), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes low-density lipoprotein receptor-related protein 8 as secreted and also located in the cell membrane. Tissue expression noted by UniProt (human): Expressed mainly in brain and placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
HuangHaofeng et al. (2025) Reelin-LRP8 signaling mediates brain dissemination of breast cancer cells via abluminal migration. EMBO Molecular Medicine. 10.1038/s44321-025-00260-0
Qian Wang et al. (2012) Effects of maternal and dietary selenium (Se-enriched yeast) on the expression of Sel P and apoER2 of germ cells of their offspring in goats. Animal Reproduction Science. 10.1016/j.anireprosci.2012.09.008
