| Field | Specification |
|---|---|
| Target | |
| Alternative names | Low-density lipoprotein receptor-related protein 8; LRP-8; Apolipoprotein E receptor 2; LRP8; APOER2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ApoER2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACFH-12 is a rabbit monoclonal antibody that recognizes low-density lipoprotein receptor-related protein 8 (gene LRP8) in human, mouse and rat samples and is validated for Western blot and immunoprecipitation. The predicted molecular weight is 105.6 kDa, and the supplier reports an observed band at about 106 kDa.
LRP8, also called ApoER2, is a member of the LDL receptor family that binds Reelin and apolipoprotein E-containing ligands. Together with the VLDL receptor it relays Reelin signals to the adaptor DAB1, guiding neuronal layering in the developing forebrain, and it also contributes to sperm maturation in the epididymis and to selenium uptake by selenoprotein P.
| Target | Low-density lipoprotein receptor-related protein 8 (gene LRP8; UniProt Q14114, human) |
|---|---|
| Clone | ACFH-12 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 105.6 kDa |
| Observed MW | 106 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunoprecipitation | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human apoER2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt describes low-density lipoprotein receptor-related protein 8 as secreted and also located in the cell membrane. Tissue expression noted by UniProt (human): Expressed mainly in brain and placenta.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
