| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein A-I; Apo-AI; ApoA-I; Apolipoprotein A1; Proapolipoprotein A-I; ProapoA-I; Truncated apolipoprotein A-I; Apolipoprotein A-I (1-242); APOA1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264). Mouse Apolipoprotein A I shares 64% and 68.6% amino acid (aa) sequence identity with human and rat Apolipoprotein A I, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17G5 is a mouse monoclonal antibody that recognizes apolipoprotein A-I (gene APOA1) in human samples and is validated for ELISA and Western blot. The predicted molecular weight is 30.8 kDa, and the supplier reports an observed band at about 25 kDa.
Apolipoprotein A-I (APOA1) is the main protein component of high-density lipoprotein (HDL). It helps remove excess cholesterol from peripheral tissues and carry it to the liver, and it activates lecithin-cholesterol acyltransferase (LCAT), which esterifies cholesterol within HDL particles. APOA1 is widely measured in studies of lipid metabolism and cardiovascular health.
| Target | Apolipoprotein A-I (gene APOA1; UniProt P02647, human) |
|---|---|
| Clone | 17G5 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG1 |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 30.8 kDa |
| Observed MW | 25 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| ELISA (capture antibody) | 1–5 µg/mL |
Conditions in the example images (WB): 12% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Apolipoprotein A I fragment (Asp25–Gln264), expressed in E. coli. Sequence identity with the human and rat orthologs: 64% and 68.6%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein A-I as a secreted protein. Tissue expression noted by UniProt (human): Major protein of plasma HDL, also found in chylomicrons.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Gang Liu et al. (2026) PVA modification mitigates PVDF hydrophobicity for concurrent protein and glycan analysis. Analytica Chimica ACTA. 10.1016/j.aca.2026.345230
