| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein B-100;Apo B-100;Apolipoprotein B-48;Apo B-48;APOB; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Apolipoprotein B recombinant protein (Position: Q246-N450). Human Apolipoprotein B shares 82.4% and 80.5% amino acid (aa) sequence identity with mouse and rat Apolipoprotein B, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects apolipoprotein B-100 (gene APOB) in human samples and is validated for IHC and Western blot. The predicted molecular weight is 515.6 kDa, and the supplier reports an observed band at about 250 kDa.
Apolipoprotein B (APOB) is the core structural protein of atherogenic lipoproteins. The full-length form, apo B-100, is made in the liver and found on VLDL and LDL, where it serves as the ligand for the LDL receptor, while the shorter apo B-48 is made in the intestine for chylomicrons. APOB is a central marker in cholesterol and cardiovascular research.
| Target | Apolipoprotein B-100 (gene APOB; UniProt P04114, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 515.6 kDa |
| Observed MW | 250 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
|---|---|
| Western blot | 0.1–0.5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Apolipoprotein B fragment (Gln246–Asn450), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 82.4% and 80.5%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein B-100 as secreted and also located in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wu Xingjie et al. (2026) In-situ polymerization-mediated glycan density measurement on extracellular vesicle surface for acute myeloid leukemia diagnosis. Journal of Nanobiotechnology. 10.1186/s12951-026-04400-7
Ti Qin et al. (2023) Size-Exclusion Chromatography-Based Extracellular Vesicles Size Subtyping and Multiplex Membrane Protein Profiling for Differentiating Gastrointestinal Cancer Prognosis. Analyst. 10.1039/D3AN01027A
