| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein C-III;Apo-CIII;ApoC-III;Apolipoprotein C3;APOC3; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human Apolipoprotein CIII recombinant protein (Position: S21-A99). Human Apolipoprotein CIII shares 55.3% and 54.3% amino acid (aa) sequence identity with mouse and rat Apolipoprotein CIII, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets apolipoprotein C-III (gene APOC3) in human and mouse samples and is validated for Western blot, IHC and flow cytometry.
Apolipoprotein C-III (APOC3) is a component of VLDL and HDL that raises plasma triglyceride levels. It inhibits lipoprotein lipase and slows the uptake of triglyceride-rich lipoprotein remnants by the liver, which has made it an important target in studies of triglyceride metabolism and cardiovascular risk.
| Target | Apolipoprotein C-III (gene APOC3; UniProt P02656, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse) |
| Flow cytometry | 1–3 µg/1×106 cells (Human) |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human Apolipoprotein CIII fragment (Ser21–Ala99), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 55.3% and 54.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt describes apolipoprotein C-III as a secreted protein. Tissue expression noted by UniProt (human): Liver.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuan Lin et al. (2023) Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy. Journal of Proteome Research. 10.1021/acs.jproteome.2c00756
Huali Chen et al. (2020) iTRAQ‑based quantitative proteomics analysis of the potential application of secretoneurin gene therapy for cardiac hypertrophy induced by DL‑isoproterenol hydrochloride in mice. International Journal of Molecular Medicine. 10.3892/ijmm.2020.4472
