| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apolipoprotein F; Apo-F; Lipid transfer inhibitor protein; LTIP; APOF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Apolipoprotein F |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21A70 is a rabbit monoclonal antibody that recognizes apolipoprotein F (gene APOF) in human samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 35.4 kDa, and the supplier reports an observed band at about 33 kDa.
Apolipoprotein F (APOF) is a minor plasma apolipoprotein that associates mainly with LDL and, to a lesser extent, with VLDL and HDL. It inhibits cholesteryl ester transfer protein (CETP), so it is studied as a regulator of how cholesteryl esters are exchanged between lipoprotein classes.
| Target | Apolipoprotein F (gene APOF; UniProt Q13790, human) |
|---|---|
| Clone | 21A70 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 35.4 kDa |
| Observed MW | 33 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human plasma cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human apolipoprotein F.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes apolipoprotein F as a secreted protein. Tissue expression noted by UniProt (human): Expressed by the liver and secreted in plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
