| Field | Specification |
|---|---|
| Target | |
| Alternative names | NEDD8-activating enzyme E1 regulatory subunit;Amyloid beta precursor protein-binding protein 1, 59 kDa;APP-BP1;Amyloid protein-binding protein 1;Proto-oncogene protein 1;NAE1;APPBP1;HPP1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human APPBP1 recombinant protein (Position: E181-L534). Human APPBP1 shares 98% amino acid (aa) sequence identity with mouse APPBP1. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects NEDD8-activating enzyme E1 regulatory subunit (gene NAE1) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 60.2 kDa, and the supplier reports an observed band at about 60 kDa.
NAE1 (APPBP1) is the regulatory subunit of the NEDD8-activating enzyme, the E1 that works with UBA3 to start the neddylation cascade. Neddylation activates cullin-RING ubiquitin ligases, which control the turnover of many cell cycle and signaling proteins. The NAE complex is the target of the inhibitor pevonedistat (MLN4924) in cancer research.
| Target | NEDD8-activating enzyme E1 regulatory subunit (gene NAE1; UniProt Q13564, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 60.2 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human SH-SY5Y cells, human U251 cells, human U2OS cells, rat brain tissue, mouse brain tissue, mouse kidney tissue lysates.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant human APPBP1 fragment (Glu181–Leu534), expressed in E. coli. Sequence identity with the mouse ortholog: 98%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places NEDD8-activating enzyme E1 regulatory subunit in the cell membrane. Tissue expression noted by UniProt (human): Ubiquitous in fetal tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
