| Field | Specification |
|---|---|
| Target | |
| Alternative names | DCC-interacting protein 13-alpha; Dip13-alpha; Adapter protein containing PH domain, PTB domain and leucine zipper motif 1; APPL1; APPL; DIP13A; KIAA1428 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human APPL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 18A86 is a rabbit monoclonal antibody that recognizes DCC-interacting protein 13-alpha (gene APPL1) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 79.7 kDa, and the supplier reports an observed band at about 85 kDa.
APPL1 (adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1) is an adaptor located on a subset of early endosomes. It binds membrane receptors and signaling proteins, including the adiponectin receptors and AKT, and is studied in insulin and adiponectin signaling, endosomal trafficking and cell proliferation.
| Target | DCC-interacting protein 13-alpha (gene APPL1; UniProt Q9UKG1, human) |
|---|---|
| Clone | 18A86 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 79.7 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human APPL.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places DCC-interacting protein 13-alpha in the early endosome membrane, nucleus, cytoplasm and endosome. Tissue expression noted by UniProt (human): High levels in heart, ovary, pancreas and skeletal muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
