Anti-AQP11 Antibody Picoband®

Overview

Anti-AQP11 Antibody Picoband® is an antibody for AQP11 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human,Mouse,Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: AQP11 (interleukin 17B); UniProt: Q8NBQ7
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 53 kDa
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-AQP11 Antibody Picoband® catalog # A11399.

Biological background

Biological context: Stimulates the release of tumor necrosis factor alpha and IL-1-beta from the monocytic cell line THP-1. .

Expression and localization notes: cellular localization: Secreted., tissue context: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary..

Common research applications

• Western blotting (WB): Compare AQP11 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of AQP11 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify AQP11-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Background: AQP11 has a unique asparagine-proline-alanine (NPA) box distinct from those of other AQPs, suggesting a different pore structure and function. Using Northern blot analysis, they detected highest expression of mouse Aqp11 in testis, followed by liver and kidney. Expression was much weaker in heart, brain, and muscle. Western blot analysis of mouse kidney membrane fractions detected Aqp11 at an apparent molecular mass of 26 kD, lower than the calculated molecular mass of 30 kD. Immunohistochemical analysis localized Aqp11 to mouse renal proximal tubule cells, where it showed a perinuclear distribution. Fluorescence-tagged Aqp11 localized with an endoplasmic reticulum marker.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: Secreted.
• Tissue details: Expressed in adult pancreas, small intestine, stomach, spinal cord and testis. Less pronounced expression in prostate, colon mucosal lining, and ovary.
• Research category: Cytokines,Immunology,Innate Immunity,Interleukins

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.