| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aquaporin-3;AQP-3;Aquaglyceroporin-3;Aqp3; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of mouse Aquaporin 3, different from the related human sequence by three amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes aquaporin-3 (gene Aqp3) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 31.6 kDa, and the supplier reports an observed band at about 32 kDa.
Aquaporin-3 is an aquaglyceroporin, a membrane channel that carries glycerol and hydrogen peroxide as well as water. It is prominent in kidney collecting duct cells, airway epithelium and epidermal keratinocytes, where glycerol transport supports skin hydration and barrier function, and it is studied in skin biology, kidney physiology and cancer.
| Target | Aquaporin-3 (gene Aqp3; UniProt Q8R2N1, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 31.6 kDa |
| Observed MW | 32 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat, Human) |
|---|
Samples with a confirmed band (WB): mouse kidney tissue, mouse brain tissue, rat kidney tissue, rat brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the middle region of mouse aquaporin 3. It differs from the human sequence at three residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aquaporin-3 in the cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (mouse): Detected in principal cells in collecting ducts in kidney medulla (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Zhengxu Zhang et al. (2025) Effects of Mume Fructus extract on smoking-induced cough and TNBS-induced colitis in rats and its underlying mechanisms based on gut-lung body fluid metabolism. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120539
