| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aquaporin-5; AQP-5; AQP5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human Aquaporin 5, which shares 71.7% and 73% amino acid (aa) sequence identity with mouse and rat Aquaporin 5, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets aquaporin-5 (gene AQP5) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 28.3 kDa, and the supplier reports an observed band at about 28 kDa.
Aquaporin-5 (AQP5) is a water channel found in secretory glands and epithelia, including salivary, lacrimal and sweat glands and lung alveolar cells. It supports water movement needed for fluid secretion and is studied in glandular function, lung biology and salivary gland dysfunction.
| Target | Aquaporin-5 (gene AQP5; UniProt P55064, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 28.3 kDa |
| Observed MW | 28 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): mouse lung tissue, rat lung tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Synthetic peptide from the C-terminal region of human aquaporin 5. Sequence identity with the mouse and rat orthologs: 71.7% and 73%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aquaporin-5 in the apical cell membrane, cell membrane and cytoplasmic vesicle membrane. Tissue expression noted by UniProt (human): Detected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Ni Zixin et al. (2024) Influenza virus uses mGluR2 as an endocytic receptor to enter cells. Nature Microbiology. 10.1038/s41564-024-01713-x
Zhengxu Zhang et al. (2025) Effects of Mume Fructus extract on smoking-induced cough and TNBS-induced colitis in rats and its underlying mechanisms based on gut-lung body fluid metabolism. Journal of Ethnopharmacology. 10.1016/j.jep.2025.120539
