| Field | Specification |
|---|---|
| Target | |
| Alternative names | ADP-ribosylation factor 5; ARF5 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ARF5 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A23 is a rabbit monoclonal antibody that recognizes ADP-ribosylation factor 5 (gene ARF5) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 20.5 kDa, and the supplier reports an observed band at about 18 kDa.
ADP-ribosylation factor 5 (ARF5) is a class II ARF small GTPase involved in membrane trafficking. It contributes to vesicle formation at the Golgi apparatus and has been linked to regulation of mTORC1 signaling at the plasma membrane, and it is studied in protein transport and endosomal traffic.
| Target | ADP-ribosylation factor 5 (gene ARF5; UniProt P84085, human) |
|---|---|
| Clone | 25A23 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 20.5 kDa |
| Observed MW | 18 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ARF5.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ADP-ribosylation factor 5 in the cell membrane, Golgi apparatus, cytoplasm and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
