| Field | Specification |
|---|---|
| Target | |
| Alternative names | ADP-ribosylation factor-related protein 1; ARF-related protein 1; ARP; ARFRP1; ARP1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ARFRP1 / ARP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 28A57 is a rabbit monoclonal antibody that recognizes ADP-ribosylation factor-related protein 1 (gene ARFRP1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 22.6 kDa, and the supplier reports an observed band at about 23 kDa.
ARF-related protein 1 (ARFRP1) is a small GTPase located at the trans-Golgi network. It directs ARL1 and its golgin effectors to the trans-Golgi to control protein sorting, and it is needed for proper lipidation of chylomicrons in the intestine and VLDL in the liver.
| Target | ADP-ribosylation factor-related protein 1 (gene ARFRP1; UniProt Q13795, human) |
|---|---|
| Clone | 28A57 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 22.6 kDa |
| Observed MW | 23 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ARFRP1 / ARP.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ADP-ribosylation factor-related protein 1 in the Golgi apparatus. Tissue expression noted by UniProt (human): Found in most tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
