| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arginase-2, mitochondrial; Arginase II; Kidney-type arginase; Non-hepatic arginase; Type II arginase; ARG2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Arg2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A60 is a rabbit monoclonal antibody that recognizes arginase-2, mitochondrial (gene ARG2) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 38.6 kDa, and the supplier reports an observed band at about 39 kDa.
Arginase-2 (ARG2) is a mitochondrial arginase that converts L-arginine into L-ornithine and urea outside the liver urea cycle. It is found in kidney and other tissues, where it supports ornithine production and can limit nitric oxide synthesis by competing for arginine.
| Target | Arginase-2, mitochondrial (gene ARG2; UniProt P78540, human) |
|---|---|
| Clone | 25A60 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.6 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human arg2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places arginase-2, mitochondrial in the mitochondrion. Tissue expression noted by UniProt (human): Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yu Yue et al. (2021) The Therapeutic Effects of Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells on Scleroderma. Tissue Engineering and Regenerative Medicine. 10.1007/s13770-021-00405-5
