| Field | Specification |
|---|---|
| Target | |
| Alternative names | Argininosuccinate lyase;ASAL;4.3.2.1;Arginosuccinase;ASL; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Argininosuccinate Lyase/ASL, which shares 97.4% and 92.1% amino acid (aa) sequence identity with mouse and rat Argininosuccinate Lyase/ASL, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes argininosuccinate lyase (gene ASL) in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 51.7 kDa, and the supplier reports an observed band at about 52 kDa.
Argininosuccinate lyase (ASL) is a urea cycle enzyme that cleaves argininosuccinate into arginine and fumarate. It is needed for ammonia detoxification in the liver and for arginine supply to nitric oxide synthesis in other tissues, and its deficiency causes argininosuccinic aciduria.
| Target | Argininosuccinate lyase (gene ASL; UniProt P04424, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 51.7 kDa |
| Observed MW | 52 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL |
|---|
Samples with a confirmed band (WB): rat liver tissue, rat kidney tissue, rat lung tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human argininosuccinate Lyase/ASL. Sequence identity with the mouse and rat orthologs: 97.4% and 92.1%, respectively.
Reactivity Notes
The supplier lists reactivity with mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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