| Field | Specification |
|---|---|
| Target | |
| Alternative names | Protein argonaute-2 ;Argonaute2 ;hAgo2;3.1.26.n2 ;Argonaute RISC catalytic component 2;Eukaryotic translation initiation factor 2C 2 ;eIF-2C 2 ;eIF2C 2 ;PAZ Piwi domain protein;PPD;Protein slicer ;AGO2;EIF2C2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Argonaute 2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AODE-1 is a rabbit monoclonal antibody that recognizes protein argonaute-2 (gene AGO2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 97.2 kDa, and the supplier reports an observed band at about 97 kDa.
Argonaute-2 (AGO2) is the core of the RNA-induced silencing complex (RISC). Guided by a bound microRNA or siRNA, it finds complementary mRNAs and either cleaves them through its endonuclease (slicer) activity or represses their translation, making it central to RNA interference and microRNA research.
| Target | Protein argonaute-2 (gene AGO2; UniProt Q9UKV8, human) |
|---|---|
| Clone | AODE-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 97.2 kDa |
| Observed MW | 97 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human argonaute 2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places protein argonaute-2 in the cytoplasm and nucleus.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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