| Field | Specification |
|---|---|
| Target | |
| Alternative names | Rho GTPase-activating protein 45; ARHGAP45; HMHA1; KIAA0223 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ARHGAP45 recombinant protein (Position: D111-E349). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes rho GTPase-activating protein 45 (gene ARHGAP45) in human samples and is validated for Western blot, IHC, flow cytometry and ELISA. The predicted molecular weight is 124.6 kDa, and the supplier reports an observed band at about 160 kDa.
Rho GTPase-activating protein 45 (ARHGAP45, also called HMHA1) contains a RhoGAP domain that can limit Rho GTPase activity and cytoskeletal remodeling. It is best known as the source of the minor histocompatibility antigen HA-1, which is presented by MHC molecules to T cells and is studied in stem cell transplantation immunology.
| Target | Rho GTPase-activating protein 45 (gene ARHGAP45; UniProt Q92619, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 124.6 kDa |
| Observed MW | 160 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human Raji cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human B lymphocytic tumor tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with a confirmed signal (flow cytometry): SiHa cells.
Immunogen
Recombinant human ARHGAP45 fragment (Asp111–Glu349), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places rho GTPase-activating protein 45 in the cytoplasm and cell projection. Tissue expression noted by UniProt (human): Expressed on cells of the hematopoietic lineage. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
