| Field | Specification |
|---|---|
| Target | |
| Alternative names | AT-rich interactive domain-containing protein 1A;ARID domain-containing protein 1A;B120;BRG1-associated factor 250;BAF250;BRG1-associated factor 250a;BAF250A;Osa homolog 1;hOSA1;SWI-like protein;SWI/SNF complex protein p270;SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1;hELD;ARID1A;BAF250, BAF250A, C1orf4, OSA1, SMARCF1; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ARID1A, identical to the related mouse sequence. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets AT-rich interactive domain-containing protein 1A (gene ARID1A) in human and mouse samples and is validated for flow cytometry, ICC/IF and Western blot. The predicted molecular weight is 242.0 kDa, and the supplier reports an observed band at about 250–270 kDa.
ARID1A (BAF250A) is a large DNA-binding subunit of the SWI/SNF (BAF) chromatin remodeling complex. It helps the complex reposition nucleosomes to activate or repress target genes, and it is one of the most frequently mutated chromatin regulators in human cancers, including ovarian clear cell carcinoma.
| Target | AT-rich interactive domain-containing protein 1A (gene ARID1A; UniProt O14497, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 242.0 kDa |
| Observed MW | 250–270 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human K562 cells, human THP-1 cells, human Jurkat cells, human SH-SY5Y cells, mouse testis tissue, mouse thymus tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): A549 cells.
Samples with a confirmed signal (flow cytometry): HeLa cells.
Immunogen
Synthetic peptide from the middle region of human ARID1A. The peptide sequence is conserved in mouse.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places AT-rich interactive domain-containing protein 1A in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
