| Field | Specification |
|---|---|
| Target | |
| Alternative names | AT-rich interactive domain-containing protein 1A; ARID domain-containing protein 1A; B120; BRG1-associated factor 250; BAF250; BRG1-associated factor 250a; BAF250A; Osa homolog 1; hOSA1; SWI-like protein; SWI/SNF complex protein p270; SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatin subfamily F member 1; hELD; ARID1A; C1orf4; OSA1; SMARCF1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ARID1A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 17A27 is a rabbit monoclonal antibody that recognizes AT-rich interactive domain-containing protein 1A (gene ARID1A) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 242.0 kDa, and the supplier reports an observed band at about 242 kDa.
ARID1A (BAF250A) is a large DNA-binding subunit of the SWI/SNF (BAF) chromatin remodeling complex. It helps the complex reposition nucleosomes to activate or repress target genes, and it is one of the most frequently mutated chromatin regulators in human cancers, including ovarian clear cell carcinoma.
| Target | AT-rich interactive domain-containing protein 1A (gene ARID1A; UniProt O14497, human) |
|---|---|
| Clone | 17A27 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 242.0 kDa |
| Observed MW | 242 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Conditions in the example images (WB): 8% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human adenocarcinoma of colon tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Samples with confirmed staining (ICC/IF): SH-SY5Y cells.
Immunogen
Synthetic peptide based on the sequence of human ARID1A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places AT-rich interactive domain-containing protein 1A in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and PBL, and at a much lower level in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mei Liu et al. (2025) Propofol Inhibits the Stem Characteristics and Tumor Growth of NSCLC by Altering the Expression and Localization of HDAC1. FASEB Journal. 10.1096/fj.202501034R
