| Field | Specification |
|---|---|
| Target | |
| Alternative names | ARID3A; DRIL1; DRIL3; DRX; E2FBP1; AT-rich interactive domain-containing protein 3A; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; Bright; Dead ringer-like protein 1; E2F-binding protein 1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ARID3A, which shares 95.8% amino acid (aa) sequence identity with mouse ARID3A. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects AT-rich interactive domain-containing protein 3A (gene ARID3A) in human samples and is validated for Western blot, flow cytometry and immunoprecipitation.
ARID3A (also called Bright or DRIL1) is a transcription factor of the AT-rich interaction domain family. It binds AT-rich DNA elements to regulate gene expression and is studied in B-cell differentiation, immunoglobulin gene transcription and cell cycle control.
| Target | AT-rich interactive domain-containing protein 3A (gene ARID3A; UniProt Q99856, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Immunogen
Synthetic peptide from the N-terminal region of human ARID3A. Sequence identity with the mouse ortholog: 95.8%.
Reactivity Notes
The supplier lists reactivity with human. UniProt places AT-rich interactive domain-containing protein 3A in the nucleus and cytoplasm. Tissue expression noted by UniProt (human): Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
