| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | DAP3-binding cell death enhancer 1; Death ligand signal enhancer; DELE1; DELE; KIAA0141 |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E.coli-derived human ARID5A recombinant protein (Position: R55-L594). |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
Anti-ARID5A Antibody Picoband® is an antibody for ARID5A detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Human. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.
Key elements and design rationale
- Target: ARID5A (DAP3 binding cell death enhancer 1); UniProt: Q03989
- Antibody format: Rabbit, Polyclonal, Rabbit IgG
- Molecular weight: 64 kDa, calculated 64099 MW
- Applications: WB, IHC, Flow Cytometry, ELISA
Vendor description (summary): Boster Bio Anti-ARID5A Antibody Picoband® catalog # A14678-1.
Biological background
Biological context: Essential for the induction of death receptor-mediated apoptosis through the regulation of caspase activation.
Expression and localization notes: cellular localization: Mitochondrion., tissue context: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon..
Common research applications
- Western blotting (WB): Compare ARID5A levels across samples and conditions using appropriate loading and biological controls.
- Immunohistochemistry (IHC): Evaluate spatial distribution of ARID5A in tissue sections, considering fixation and antigen retrieval effects.
- Flow cytometry: Quantify ARID5A-positive populations in single-cell suspensions with appropriate gating and controls.
- ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.
Notes for experimental interpretation
- Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
- Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.
Additional product notes (from provided fields)
- Background: Members of the ARID protein family, including ARID5A, have diverse functions but all appear to play important roles in development, tissue-specific gene expression, and regulation of cell growth.
- Cross reactivity: No cross-reactivity with other proteins.
- Cellular localization: Mitochondrion.
- Tissue details: Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
- Research category: Signal Transduction
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
