| Field | Specification |
|---|---|
| Target | |
| Alternative names | Actin-related protein 2/3 complex subunit 2; Arp2/3 complex 34 kDa subunit; p34-ARC; ARPC2; ARC34 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ARPC2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27A24 is a rabbit monoclonal antibody that recognizes actin-related protein 2/3 complex subunit 2 (gene ARPC2) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 34.3 kDa, and the supplier reports an observed band at about 34 kDa.
ARPC2 (p34-Arc) is a core structural subunit of the Arp2/3 complex, which nucleates branched actin filaments when activated by nucleation-promoting factors such as WASP and WAVE. It is studied in cell migration, lamellipodia formation and other actin-driven processes.
| Target | Actin-related protein 2/3 complex subunit 2 (gene ARPC2; UniProt O15144, human) |
|---|---|
| Clone | 27A24 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 34.3 kDa |
| Observed MW | 34 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human 293T cells, human HeLa cells, human Jurkat cells, human MCF-7 cells, rat skeletal muscle tissue, rat PC-12 cells, mouse skeletal muscle tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ARPC2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places actin-related protein 2/3 complex subunit 2 in the cytoplasm, cell projection, synapse and nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
