| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arylsulfatase A; ASA; Cerebroside-sulfatase; Arylsulfatase A component B; Arylsulfatase A component C; ARSA |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ARSA, different from the related mouse sequence by six amino acids. |
| Molecular weight | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 4C10 is a mouse monoclonal antibody that recognizes arylsulfatase A (gene ARSA) in human, mouse and rat samples and is validated for flow cytometry, IHC and Western blot. The predicted molecular weight is 53.6 kDa, and the supplier reports an observed band at about 54 kDa.
Arylsulfatase A (ARSA) is a lysosomal enzyme that removes the sulfate group from sulfatides (cerebroside-3-sulfate), key lipids of myelin. Deficiency of ARSA causes metachromatic leukodystrophy, in which sulfatides accumulate and damage the nervous system.
| Target | Arylsulfatase A (gene ARSA; UniProt P15289, human) |
|---|---|
| Clone | 4C10 |
| Host / Clonality / Isotype | Mouse / Monoclonal / Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3 |
| Calculated MW | 53.6 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human, Mouse) |
Samples with a confirmed band (WB): rat testis tissue, rat liver tissue, rat brain tissue, rat lung tissue, mouse testis tissue, mouse liver tissue, mouse brain tissue, mouse lung tissue, mouse HEPA1–6 cells, human A375 cells, human A549 cells, human SMMC-7721 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Samples with a confirmed signal (flow cytometry): ANA-1 cells, Raji cells.
Immunogen
Synthetic peptide from the C-terminal region of human ARSA. It differs from the mouse sequence at six residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places arylsulfatase A in the endoplasmic reticulum and lysosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
