| Field | Specification |
|---|---|
| Target | |
| Alternative names | Set1/Ash2 histone methyltransferase complex subunit ASH2;ASH2-like protein;ASH2L;ASH2L1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ASH2L |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EOB-1 is a rabbit monoclonal antibody that recognizes set1/Ash2 histone methyltransferase complex subunit ASH2 (gene ASH2L) in human, mouse and rat samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 68.7 kDa; the supplier reports observed bands at 69, 90 kDa.
ASH2L is a core subunit of the SET1/MLL (COMPASS-like) histone methyltransferase complexes, together with WDR5, RBBP5 and DPY30. These complexes methylate histone H3 at lysine 4, a mark associated with active gene promoters, and ASH2L is studied in transcriptional regulation and development.
| Target | Set1/Ash2 histone methyltransferase complex subunit ASH2 (gene ASH2L; UniProt Q9UBL3, human) |
|---|---|
| Clone | EOB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.7 kDa |
| Observed MW | 69, 90 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Samples with confirmed staining (IHC): human kideny tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human ASH2L.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places set1/Ash2 histone methyltransferase complex subunit ASH2 in the nucleus. Tissue expression noted by UniProt (human): Ubiquitously expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
