| Field | Specification |
|---|---|
| Target | |
| Alternative names | Aspartate aminotransferase, cytoplasmic; cAspAT; Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A; GOT1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Aspartate Aminotransferase. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADEA-7 is a rabbit monoclonal antibody that recognizes aspartate aminotransferase, cytoplasmic (gene GOT1) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 46.2 kDa, and the supplier reports an observed band at about 41 kDa.
Cytoplasmic aspartate aminotransferase (GOT1) transfers amino groups between aspartate and α-ketoglutarate to form oxaloacetate and glutamate. As part of the malate-aspartate shuttle it moves reducing equivalents between cytosol and mitochondria, and it also helps regulate glutamate levels, so it is studied in amino acid and energy metabolism.
| Target | Aspartate aminotransferase, cytoplasmic (gene GOT1; UniProt P17174, human) |
|---|---|
| Clone | ADEA-7 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 46.2 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human MCF-7 cells, human K562 cells, human HepG2 cells, human A431 cells, rat heart tissue, rat liver tissue, mouse heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human aspartate Aminotransferase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places aspartate aminotransferase, cytoplasmic in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
