Anti-ASS1 Rabbit Monoclonal Antibody

Overview

This product is an anti-ASS1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone 23A63; isotype IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC, ICC, IF, IP, Flow (as provided in the source record). Boster Bio Anti-ASS1 Rabbit Monoclonal Antibody catalog # M02212-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Key elements and design rationale

• Target: ASS1 (Caspase-10).
• Antibody format: Monoclonal; clone 23A63; isotype IgG.
• Host: Rabbit.
• Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).

This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.

Biological background

ASS1 (protein: Lysosome-associated membrane glycoprotein 2 (Lamp2)) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase- 3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. . Reported cellular localization context: Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit . Cell projection, pseudopodium . Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity). . Tissue expression notes (as provided): Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Research relevance and current trends

• Research context keywords from the source record include: Adaptive Immunity,Alzheimer's Disease,Cancer,Cell Biology,Cell Cycle,Cell Division,Chromatin Remodeling,Chromosome Structure,Epigenetics and Nuclear Signaling,ER Proteins,Immunology,Neurodegenerative Disease,Neurology Process,Neuroscience,Oncoproteins,Oncoproteins/Suppressors,Protein Phosphorylation,Protein Trafficking,Ser/Thr Phosphatases,Signal Transduction,T Cells.
• Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
• Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.

Common research applications

• Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
• Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
• Immunofluorescence/ICC (IF/ICC): visualize subcellular localization patterns and cell-to-cell heterogeneity.
• Flow cytometry: quantify target-positive populations and compare shifts in marker distributions.
• Immunoprecipitation (IP): enrich target complexes for downstream immunoblot or interaction analyses.

Workflow ideas (metafield): Validate ASS1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ASS1 expression by Western blot in cell or tissue lysates, Detect ASS1 in FFPE tissue sections by immunohistochemistry, Localize ASS1 by immunofluorescence/immunocytochemistry in cultured cells, Quantify ASS1-positive cells by flow cytometry in single-cell suspensions, Enrich ASS1 by immunoprecipitation from lysates for downstream analysis

Notes for experimental interpretation

• Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
• Apparent molecular weight may vary by sample type and processing (observed MW: 45 kDa; calculated MW: 58951 MW).
• Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.

Additional product details (from the source record)

• Molecular weight (observed): 45 kDa
• Cellular localization (provided): Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit . Cell projection, pseudopodium . Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs. The monomeric form is predominantly located in the nucleus. The oligomeric form is located in the cytoplasm. Translocates to the nucleus upon stimulation of OPRD1 (By similarity). .
• Tissue details (provided): Detectable in most tissues. Lowest expression is seen in brain, kidney, prostate, testis and colon.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.