| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATPase family AAA domain-containing protein 2; AAA nuclear coregulator cancer-associated protein; ANCCA; ATAD2 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATAD2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 21A07 is a rabbit monoclonal antibody that recognizes ATPase family AAA domain-containing protein 2 (gene ATAD2) in human samples and is validated for Western blot, ICC/IF and flow cytometry. The predicted molecular weight is 158.6 kDa, and the supplier reports an observed band at about 180 kDa.
ATAD2 is a nuclear protein that combines an AAA+ ATPase domain with a bromodomain that recognizes acetylated histones. It acts as a transcriptional coactivator, including for estrogen receptor target genes, and it is frequently overexpressed in cancers, where it is studied in chromatin regulation and cell proliferation.
| Target | ATPase family AAA domain-containing protein 2 (gene ATAD2; UniProt Q6PL18, human) |
|---|---|
| Clone | 21A07 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 158.6 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Immunogen
Synthetic peptide based on the sequence of human ATAD2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places ATPase family AAA domain-containing protein 2 in the nucleus. Tissue expression noted by UniProt (human): Highly expressed in estrogen receptor positive breast tumors and in osteosarcoma tumors.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Shenghua Zhuo et al. (2025) Druggable target ATAD2 enhances the malignant progression and cooperates with E2F1 to up-regulate PDK1 expression in glioma. Genes & Diseases. 10.1016/j.gendis.2025.101810
