| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arginyl-tRNA--protein transferase 1; Arginyltransferase 1; R-transferase 1; Arginine-tRNA--protein transferase 1; ATE1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30A62 is a rabbit monoclonal antibody that recognizes arginyl-tRNA--protein transferase 1 (gene ATE1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 59.1 kDa, and the supplier reports an observed band at about 59 kDa.
Arginyl-tRNA-protein transferase 1 (ATE1) adds arginine to the N-terminus of proteins bearing an exposed aspartate or glutamate. This arginylation can mark proteins for degradation through the N-degron pathway and can also change their function, and ATE1 is studied in protein turnover, cytoskeleton regulation and cardiovascular development.
| Target | Arginyl-tRNA--protein transferase 1 (gene ATE1; UniProt O95260, human) |
|---|---|
| Clone | 30A62 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.1 kDa |
| Observed MW | 59 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): HepG2 cells, mouse spleen.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ATE1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places arginyl-tRNA--protein transferase 1 in the nucleus and cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
