| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-1;cAMP-dependent transcription factor ATF-1;Activating transcription factor 1;Protein TREB36;ATF1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATF1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HBF-1 is a rabbit monoclonal antibody that recognizes cyclic AMP-dependent transcription factor ATF-1 (gene ATF1) in human samples and is validated for Western blot, ICC/IF and immunoprecipitation. The predicted molecular weight is 29.2 kDa, and the supplier reports an observed band at about 36 kDa.
Cyclic AMP-dependent transcription factor ATF-1 (ATF1) is a bZIP transcription factor closely related to CREB. It binds cAMP response elements (CRE) in gene promoters and is activated by phosphorylation, and ATF1 fusion genes are found in tumors such as clear cell sarcoma and angiomatoid fibrous histiocytoma.
| Target | Cyclic AMP-dependent transcription factor ATF-1 (gene ATF1; UniProt P18846, human) |
|---|---|
| Clone | HBF-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 29.2 kDa |
| Observed MW | 36 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ATF1.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclic AMP-dependent transcription factor ATF-1 in the nucleus. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
