Anti-ATF1 Rabbit Monoclonal Antibody

Overview

Anti-ATF1 Rabbit Monoclonal Antibody is an antibody targeting ATF1. Common applications include WB, ICC, IF, IP. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: HBF-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 92 kDa; calculated MW: 29233 MW.

Boster Bio Anti-ATF1 Rabbit Monoclonal Antibody catalog # M01600. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.

Key elements and design rationale

• Target: ATF1 — Cyclic AMP-dependent transcription factor ATF-1
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: HBF-1; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 92 kDa; Calculated: 29233 MW

Biological background

Protein function (datasheet): This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation. .

Cellular localization (datasheet): Nucleus.

Tissue details (datasheet): Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003). .

Research relevance and current trends

• Commonly studied in contexts related to Epigenetics and Nuclear Signaling,Hlh/Leucine Zipper,Transcription.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.