| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-2;cAMP-dependent transcription factor ATF-2;2.3.1.48 ;Activating transcription factor 2;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2;HB16;Histone acetyltransferase ATF2;cAMP response element-binding protein CRE-BP1;ATF2;CREB2, CREBP1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATF2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone IA-1 is a rabbit monoclonal antibody that recognizes cyclic AMP-dependent transcription factor ATF-2 (gene ATF2) in human samples and is validated for Western blot, IHC, ICC/IF and immunoprecipitation. The predicted molecular weight is 54.5 kDa, and the supplier reports an observed band at about 70 kDa.
Cyclic AMP-dependent transcription factor ATF-2 (ATF2) is a bZIP transcription factor that binds CRE sites, often as a dimer with c-Jun. It is activated by phosphorylation from stress-activated kinases such as p38 and JNK, and it is studied in stress responses, DNA damage signaling and cancer.
| Target | Cyclic AMP-dependent transcription factor ATF-2 (gene ATF2; UniProt P15336, human) |
|---|---|
| Clone | IA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 54.5 kDa |
| Observed MW | 70 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:30 |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human HUVEC cells, human K562 cells, human HEL cells, human SiHa cells, human SH-SY5Y cells, human Caco-2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): human colon cancer tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HeLa cells.
Immunogen
Synthetic peptide based on the sequence of human ATF2.
Reactivity Notes
The supplier lists reactivity with human. UniProt places cyclic AMP-dependent transcription factor ATF-2 in the nucleus, cytoplasm and mitochondrion outer membrane. Tissue expression noted by UniProt (human): Ubiquitously expressed, with more abundant expression in the brain. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
