| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cyclic AMP-dependent transcription factor ATF-4;cAMP-dependent transcription factor ATF-4;Activating transcription factor 4;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2;DNA-binding protein TAXREB67;Tax-responsive enhancer element-binding protein 67;TaxREB67;ATF4;CREB2, TXREB; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATF4 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HCI-1 is a rabbit monoclonal antibody that recognizes cyclic AMP-dependent transcription factor ATF-4 (gene ATF4) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 38.6 kDa, and the supplier reports an observed band at about 55 kDa.
Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a bZIP transcription factor at the center of the integrated stress response. Its translation increases when eIF2α is phosphorylated, and it then activates genes for amino acid synthesis and transport, antioxidant defense and, under prolonged stress, CHOP-mediated cell death.
| Target | Cyclic AMP-dependent transcription factor ATF-4 (gene ATF4; UniProt P18848, human) |
|---|---|
| Clone | HCI-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 38.6 kDa |
| Observed MW | 55 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human ATF4.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cyclic AMP-dependent transcription factor ATF-4 in the nucleus, nucleus speckle, cytoplasm and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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Yating Ling et al. (2025) Preliminary exploration of endoplasmic reticulum stress transmission in astrocytes and neurons, and its mediators. Molecular Medicine Reports. 10.3892/mmr.2025.13532
Yanying Zhang et al. (2026) Methionine promotes cell proliferation in bovine mammary epithelial cells via the ATF4-GSK3β signalling pathway. Journal of Dairy Research. 10.1017/S0022029926102416
