Anti-ATF7 Rabbit Monoclonal Antibody

SKU:BHA21007907
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Boster Bio
Boster Bio
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Overview
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Anti-ATF7 antibody (Rabbit monoclonal, clone HBD-1, Rabbit IgG). Validated for Western blot. Reactivity: Human, Mouse, Rat. Recognizes cyclic AMP-dependent transcription factor ATF-7. Observed band in Western blot: about 53 kDa.
Target ATF7
Clone number HBD-1
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB
Options selector
Catalog no. Size Conjugation
M04951 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 uL/vial — Unconjugated
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
      Form: Liquid
      Applications: WB
      Application details: WB 1:500-2000
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: refer to the option details above and the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ATF7
Alternative names Cyclic AMP-dependent transcription factor ATF-7;cAMP-dependent transcription factor ATF-7;Activating transcription factor 7;Transcription factor ATF-A;ATF7;ATFA;
Gene ID 11016
UniProt # P17544
Host Rabbit
Clone HBD-1
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen A synthesized peptide derived from human ATF7
Molecular weight 53.0 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Nucleus . Nucleus, nucleoplasm . Mainly nucleoplasmic. Restricted distribution to the perinuculear region. The sumoylated form locates to the nuclear periphery.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M04951
Main SKU BHA21007907
Rabbit Monoclonal

Product Overview

Clone HBD-1 is a rabbit monoclonal antibody that recognizes cyclic AMP-dependent transcription factor ATF-7 (gene ATF7) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 53.0 kDa, and the supplier reports an observed band at about 53 kDa.

Cyclic AMP-dependent transcription factor ATF-7 (ATF7) is a bZIP transcription factor closely related to ATF2. In unstressed cells it helps keep target genes silent by recruiting histone H3K9 methyltransferases, and stress-induced phosphorylation releases this repression, a mechanism studied in innate immune memory and stress responses.

Target Cyclic AMP-dependent transcription factor ATF-7 (gene ATF7; UniProt P17544, human)
Clone HBD-1
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 53.0 kDa
Observed MW 53 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000

Immunogen

Synthetic peptide based on the sequence of human ATF7.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places cyclic AMP-dependent transcription factor ATF-7 in the nucleus and chromosome. Tissue expression noted by UniProt (human): Expressed in various tissues including heart, brain, placenta, lung and skeletal muscle.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 53.0 kDa; observed band at about 53 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human ATF7.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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