| Field | Specification |
|---|---|
| Target | |
| Alternative names | Autophagy-related protein 16-1;APG16-like 1;ATG16L1;APG16L;UNQ9393/PRO34307; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Atg16L1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABIE-1 is a rabbit monoclonal antibody that recognizes autophagy-related protein 16-1 (gene ATG16L1) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 68.3 kDa, and the supplier reports an observed band at about 68 kDa.
ATG16L1 binds the ATG12-ATG5 conjugate and directs it to membranes, where the complex promotes lipidation of LC3 family proteins during autophagosome formation. It also supports non-canonical LC3 lipidation, and a common ATG16L1 variant is associated with Crohn's disease.
| Target | Autophagy-related protein 16-1 (gene ATG16L1; UniProt Q676U5, human) |
|---|---|
| Clone | ABIE-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.3 kDa |
| Observed MW | 68 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human THP-1 cells, human HeLa cells, human 293T cells, human Daudi cells, rat C6 cells, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1: 500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human atg16L1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places autophagy-related protein 16-1 in the cytoplasm, preautophagosomal structure membrane, endosome membrane and lysosome membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Na Zhao et al. (2022) DJ-1 activates the Atg5-Atg12-Atg16L1 complex via Sirt1 to influence microglial polarization and alleviate cerebral ischemia/reperfusion-induced inflammatory injury. Neurochemistry International. 10.1016/j.neuint.2022.105341
Li Yaling et al. (2020) Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy. Frontiers in Pharmacology. 10.3389/fphar.2020.01150
