| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Autophagy-related protein 16-1;APG16-like 1;ATG16L1;APG16L;UNQ9393/PRO34307; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human Atg16L1 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Storage | |
| Target | |
| UniProt # |
Overview
This product is an anti-ATG16L1 antibody for target detection and characterization. Key identifiers include host species: Rabbit; Monoclonal; clone ABIE-1; isotype Rabbit IgG; reactivity: Human,Mouse,Rat. Reported application contexts include WB, IHC (as provided in the source record). Boster Bio Anti-Atg16L1/Atg16 Rabbit Monoclonal Antibody catalog # M00526. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Key elements and design rationale
- Target: ATG16L1 (Autophagy-related protein 16-1).
- Antibody format: Monoclonal; clone ABIE-1; isotype Rabbit IgG.
- Host: Rabbit.
- Species reactivity: Human,Mouse,Rat (confirm in your model system with appropriate controls).
This description is intended to help interpret the antibody design and the biological context of the target using the fields provided in the catalog record, alongside general experimental considerations.
Biological background
ATG16L1 (protein: T-cell surface glycoprotein CD4) is a commonly studied target in molecular and cellular biology. Functional context (as provided): Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II. Thereby, controls the elongation of the nascent autophagosomal membrane. . Reported cellular localization context: Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. . Tissue expression notes (as provided): Plasma. Synthesized by the fetal liver and yolk sac.
Research relevance and current trends
- Research context keywords from the source record include: Cancer,Cardiovascular,Cell Biology,Cell Death,Metabolism,Metabolism Processes,Neurodegenerative Disease,Neurology Process,Neuroscience,Oncoproteins/Suppressors,Pathways and Processes,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Signal Transduction,Tumor Suppressors,Ub-Like Proteins.
- Current studies often focus on connecting target abundance/localization to pathway perturbations across models, tissues, and cell states.
- Quantitative and multiplexed assays (e.g., imaging + immunoblot panels) are commonly used to compare phenotypes across conditions and time-courses.
Common research applications
- Western blotting (WB): assess relative target abundance across samples, treatments, or time-points.
- Immunohistochemistry (IHC): evaluate spatial distribution of target-positive staining in tissue architecture.
Workflow ideas (metafield): Validate ATG16L1 antibody specificity using KO/KD control samples (WB/IF/IHC as appropriate), Detect ATG16L1 expression by Western blot in cell or tissue lysates, Detect ATG16L1 in FFPE tissue sections by immunohistochemistry
Notes for experimental interpretation
- Consider isoforms and post-translational modifications (PTMs) that may shift apparent molecular weight or epitope accessibility.
- Apparent molecular weight may vary by sample type and processing (observed MW: 68 kDa; calculated MW: 68265 MW).
- Control concepts: include appropriate negative controls (e.g., isotype, KO/KD samples) and orthogonal validation when feasible.
Additional product details (from the source record)
- Molecular weight (observed): 68 kDa
- Cellular localization (provided): Cytoplasm . Preautophagosomal structure membrane ; Peripheral membrane protein . Recruited to omegasomes membranes by WIPI2. Omegasomes are endoplasmic reticulum connected strutures at the origin of preautophagosomal structures. Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5. Localizes also to discrete punctae along the ciliary axoneme. .
- Tissue details (provided): Plasma. Synthesized by the fetal liver and yolk sac.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
