| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cysteine protease ATG4A; AUT-like 2 cysteine endopeptidase; Autophagy-related cysteine endopeptidase 2; Autophagin-2; Autophagy-related protein 4 homolog A; HsAPG4A; hAPG4A; ATG4A; APG4A; AUTL2 |
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| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
| Molecular weight | |
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| Concentration | |
| Form | Liquid |
| Storage | |
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Product Overview
Clone ACHB-1 is a rabbit monoclonal antibody that recognizes cysteine protease ATG4A in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 45.4 kDa, and the supplier reports an observed band at about 45 kDa.
ATG4A is one of four ATG4 cysteine proteases in mammals. These enzymes cleave ATG8 family proteins to expose the C-terminal glycine needed for lipidation and later remove them from membranes (delipidation); ATG4A acts preferentially on GABARAP subfamily members.
| Target | Cysteine protease ATG4A (gene ATG4A; UniProt Q8WYN0, human) |
|---|---|
| Clone | ACHB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 45.4 kDa |
| Observed MW | 45 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): human HepG2 cells, human HeLa cells, human Jurkat cells, human T-47D cells, rat brain tissue, mouse brain tissue, mouse C2C12 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human atg4A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cysteine protease ATG4A in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Hao Zhang et al. (2025) LncRNA FOXD3-AS1 modulates ER stress and epithelial barrier dysfunction in allergic rhinitis by destabilizing CHOP mRNA. Cellular Signalling. 10.1016/j.cellsig.2025.111737
