| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cysteine protease ATG4B;3.4.22.-;AUT-like 1 cysteine endopeptidase;Autophagin-1;Autophagy-related cysteine endopeptidase 1;Autophagy-related protein 4 homolog B;hAPG4B;ATG4B;APG4B, AUTL1, KIAA0943; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Atg4B |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABIA-1 is a rabbit monoclonal antibody that recognizes cysteine protease ATG4B in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 44.3 kDa, and the supplier reports an observed band at about 44 kDa.
ATG4B is the most active of the mammalian ATG4 cysteine proteases. It processes LC3 and other ATG8 family proteins so they can be lipidated onto autophagosome membranes and later recycles them by delipidation, and it is studied as a regulator of autophagy flux.
| Target | Cysteine protease ATG4B (gene ATG4B; UniProt Q9Y4P1, human) |
|---|---|
| Clone | ABIA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 44.3 kDa |
| Observed MW | 44 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human atg4B.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cysteine protease ATG4B in the cytoplasm, cytoplasmic vesicle, endoplasmic reticulum and mitochondrion.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
