| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin-like modifier-activating enzyme ATG7;ATG12-activating enzyme E1 ATG7;Autophagy-related protein 7;APG7-like;hAGP7;Ubiquitin-activating enzyme E1-like protein;ATG7;APG7L; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Atg7 (Apg7) Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ACHA-1 is a rabbit monoclonal antibody that recognizes ubiquitin-like modifier-activating enzyme ATG7 in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 78.0 kDa, and the supplier reports an observed band at about 78 kDa.
ATG7 is the E1-like activating enzyme of both ubiquitin-like conjugation systems in autophagy. It activates ATG12 for transfer to ATG5 and activates LC3 family proteins for transfer to ATG3, making it essential for autophagosome formation and a common target in autophagy knockout studies.
| Target | Ubiquitin-like modifier-activating enzyme ATG7 (gene ATG7; UniProt O95352, human) |
|---|---|
| Clone | ACHA-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.0 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): human HeLa cells, human SIHA cells, human HepG2 cells, human K562 cells, human HeLa- WT cells, human Hela-GPX4 KO cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:1000 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human atg7 (Apg7) Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ubiquitin-like modifier-activating enzyme ATG7 in the cytoplasm and preautophagosomal structure. Tissue expression noted by UniProt (human): Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yang S. J. et al. (2024) Rapamycin Regulates Lipopolysaccharide-Induced Microglial Phagocytosis In Vitro. Molecular Biology. 10.1134/S0026893324700109
