Anti-ATG7/Apg7 Rabbit Monoclonal Antibody

Overview

Anti-ATG7/Apg7 Rabbit Monoclonal Antibody is an antibody targeting ATG7. Common applications include WB, IHC, ICC, IF, IP, Flow Cytometry. Key specifications include host: Rabbit; clonality: Monoclonal; clone: Clone: FCA-1; isotype: Rabbit IgG; reactivity: Human; observed MW: 35 kDa; calculated MW: 77960 MW.

Boster Bio Anti-ATG7/Apg7 Rabbit Monoclonal Antibody catalog # M00346. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.

Key elements and design rationale

• Target: ATG7 — Ubiquitin-like modifier-activating enzyme ATG7
• Antibody format: Host: Rabbit; Clonality: Monoclonal; Clone: Clone: FCA-1; Isotype: Rabbit IgG
• Species reactivity: Human
• Molecular weight guidance: Observed: 35 kDa; Calculated: 77960 MW

Biological background

Protein function (datasheet): E1-like activating enzyme involved in the 2 ubiquitin- like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. .

Cellular localization (datasheet): Cytoplasm . Preautophagosomal structure . Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme. .

Tissue details (datasheet): Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

Research relevance and current trends

• Commonly studied in contexts related to Apoptosis,Associated Proteins,Cancer,Cardiovascular,Cell Biology,Cell Death,Energy Metabolism,Energy Transfer Pathways,Intracellular,Metabolic Signaling Pathways,Metabolism,Metabolism Processes,Mitochondrial Metabolism,Pathways and Processes,Proteasome / Ubiquitin,Proteolysis/Ubiquitin,Signal Transduction,Ub-Like Proteins.
• Supports comparative expression analysis across conditions, genotypes, or treatments when paired with appropriate controls.
• Useful for confirming target presence and subcellular distribution using orthogonal readouts (e.g., microscopy vs. immunoblotting).

Common research applications

• Western blot (WB): Compare relative target abundance and apparent size/isoforms across samples; interpret bands in light of expected MW and potential PTMs.
• Immunohistochemistry (IHC): Assess tissue distribution and cell-type patterns; interpret staining with appropriate negative controls and antigen context.
• Immunofluorescence / ICC: Visualize subcellular localization and co-localization patterns; consider fixation/permeabilization compatibility and controls.
• Flow cytometry: Quantify target-positive populations in single-cell suspensions; pair with viability and isotype/FMO controls conceptually.

Notes for experimental interpretation

• Consider isoforms, post-translational modifications, and processing that can shift apparent molecular weight or localization.
• Use appropriate positive and negative controls (e.g., KO/KD, blocking peptide, or isotype controls) to support specificity interpretation.

As a monoclonal antibody, this reagent is expected to recognize a defined epitope, which can support consistency across lots when epitope accessibility is preserved.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.