| Field | Specification |
|---|---|
| Target | |
| Alternative names | Bifunctional purine biosynthesis protein ATIC; AICAR transformylase/inosine monophosphate cyclohydrolase; ATIC; PURH |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATIC recombinant protein (Position: T37-H592). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets bifunctional purine biosynthesis protein ATIC in human, mouse and rat samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 64.6 kDa, and the supplier reports an observed band at about 65 kDa.
ATIC is a bifunctional enzyme that performs the last two steps of de novo purine biosynthesis, converting AICAR to IMP through formyltransferase and cyclohydrolase activities. It is studied in nucleotide metabolism, and its AICAR transformylase activity is a target of the antifolate methotrexate.
| Target | Bifunctional purine biosynthesis protein ATIC (gene ATIC; UniProt P31939, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 64.6 kDa |
| Observed MW | 65 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human Caco-2 cells, human HepG2 cells, human K562 cells, human HEK293 cells, human HeLa cells, rat stomach tissue, rat kidney tissue, rat testis tissue, mouse kidney tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): K562 cells.
Immunogen
Recombinant human ATIC fragment (Thr37–His592), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places bifunctional purine biosynthesis protein ATIC in the cytoplasm. Tissue expression noted by UniProt (human): Present in the heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
