| Field | Specification |
|---|---|
| Target | |
| Alternative names | Atlastin-1; ATL-1; Brain-specific GTP-binding protein; GTP-binding protein 3; GBP-3; hGBP3; Guanine nucleotide-binding protein 3; Spastic paraplegia 3 protein A; ATL1; GBP3; SPG3; SPG3A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATL1 recombinant protein (Position: E24-Q552). Human ATL1 shares 97.5% and 98.3% amino acid (aa) sequence identity with mouse and rat ATL1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes atlastin-1 (gene ATL1) in human samples and is validated for Western blot, ICC/IF, flow cytometry and ELISA.
Atlastin-1 (ATL1) is a dynamin-related GTPase anchored in the ER membrane that fuses endoplasmic reticulum tubules to build the interconnected ER network. It is highly expressed in the nervous system, and mutations in ATL1 cause hereditary spastic paraplegia (SPG3A).
| Target | Atlastin-1 (gene ATL1; UniProt Q8WXF7, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human ATL1 fragment (Glu24–Gln552), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 97.5% and 98.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places atlastin-1 in the endoplasmic reticulum membrane, Golgi apparatus membrane and cell projection. Tissue expression noted by UniProt (human): Expressed predominantly in the adult and fetal central nervous system.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
