| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-citrate synthase;2.3.3.8;ATP-citrate (pro-S-)-lyase;ACL;Citrate cleavage enzyme;ACLY; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ATP citrate lyase recombinant protein (Position: M1-I180). Human ATP citrate lyase shares 95% amino acid (aa) sequence identity with both mouse and rat ATP citrate lyase. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects ATP-citrate synthase (gene ACLY) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 120.8 kDa, and the supplier reports an observed band at about 125 kDa.
ATP-citrate lyase (ACLY) splits citrate into acetyl-CoA and oxaloacetate in the cytosol. This supplies acetyl-CoA for fatty acid and cholesterol synthesis and for histone acetylation, and ACLY is the target of the cholesterol-lowering drug bempedoic acid.
| Target | ATP-citrate synthase (gene ACLY; UniProt P53396, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 120.8 kDa |
| Observed MW | 125 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 1–2 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human A549 cells, human MOLT4 cells, human U87 cells, human U251 cells, human 293T cells, human HeLa cells, human T47D cells, human HepG2 cells, rat pancreas tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human colonic adenocarcinoma tissue, human hepatocellular carcinoma tissue, human laryngeal squamous cells carcinoma tissue, human placenta tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): A594 cells.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human ATP citrate lyase fragment (Met1–Ile180), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-citrate synthase in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Guiyan Liu et al. (2024) The combination of gemcitabine and albumin-bound paclitaxel effectively inhibits de novo lipogenesis in pancreatic cancer cells by targeting the AMPK/SREBP1 pathway. Biochemical Pharmacology. 10.1016/j.bcp.2024.116721
Dong Sun et al. (2025) GCN2 promotes fatty acid metabolism through the activation of PI3K/AKT signaling in gastric cancer. Cellular Signalling. 10.1016/j.cellsig.2025.111984
Donglei Sun et al. (2020) Fat mass and obesity-associated protein regulates lipogenesis via m6A modification in fatty acid synthase mRNA. Cell Biology International. 10.1002/cbin.11490
