| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/potassium-transporting ATPase subunit alpha-2; Na(+)/K(+) ATPase alpha-2 subunit; Sodium pump subunit alpha-2; ATP1A2; KIAA0778 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATP1A2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A95 is a rabbit monoclonal antibody that recognizes sodium/potassium-transporting ATPase subunit alpha-2 (gene ATP1A2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 112.3 kDa, and the supplier reports an observed band at about 102 kDa.
ATP1A2 is the α2 catalytic subunit of the Na+/K+-ATPase, expressed mainly in astrocytes, skeletal muscle and heart. In astrocytes it helps clear potassium and glutamate from synapses, and mutations cause familial hemiplegic migraine type 2.
| Target | Sodium/potassium-transporting ATPase subunit alpha-2 (gene ATP1A2; UniProt P50993, human) |
|---|---|
| Clone | 25A95 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 112.3 kDa |
| Observed MW | 102 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human fetal heart.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ATP1A2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium/potassium-transporting ATPase subunit alpha-2 in the membrane and cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
