| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/potassium-transporting ATPase subunit alpha-3; Na(+)/K(+) ATPase alpha-3 subunit; Na(+)/K(+) ATPase alpha(III) subunit; Sodium pump subunit alpha-3; ATP1A3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATP1A3 recombinant protein (Position: D24-D583). Human ATP1A3 shares 99.8% amino acid (aa) sequence identity with both mouse and rat ATP1A3. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets sodium/potassium-transporting ATPase subunit alpha-3 (gene ATP1A3) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA.
ATP1A3 is the neuron-specific α3 catalytic subunit of the Na+/K+-ATPase. It helps restore ion gradients after intense neuronal firing, and mutations in ATP1A3 cause several neurological disorders, including rapid-onset dystonia-parkinsonism and alternating hemiplegia of childhood.
| Target | Sodium/potassium-transporting ATPase subunit alpha-3 (gene ATP1A3; UniProt P13637, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Mouse, Rat) |
| ELISA | 0.1–0.5 µg/mL |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ATP1A3 fragment (Asp24–Asp583), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 99.8%.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium/potassium-transporting ATPase subunit alpha-3 in the cell membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
