| Field | Specification |
|---|---|
| Target | |
| Alternative names | Sodium/potassium-transporting ATPase subunit beta-3; Sodium/potassium-dependent ATPase subunit beta-3; ATPB-3; CD antigen CD298; ATP1B3 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATP1B3 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22A06 is a rabbit monoclonal antibody that recognizes sodium/potassium-transporting ATPase subunit beta-3 (gene ATP1B3) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 31.5 kDa, and the supplier reports an observed band at about 39 kDa.
ATP1B3 is the β3 subunit of the Na+/K+-ATPase. Like other β subunits it is needed for the folding and plasma membrane delivery of the catalytic α subunit, and it is also known as CD298.
| Target | Sodium/potassium-transporting ATPase subunit beta-3 (gene ATP1B3; UniProt P54709, human) |
|---|---|
| Clone | 22A06 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 31.5 kDa |
| Observed MW | 39 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): mouse brain.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ATP1B3.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places sodium/potassium-transporting ATPase subunit beta-3 in the apical cell membrane, basolateral cell membrane and melanosome. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
