| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP synthase subunit alpha, mitochondrial;ATP5A1;ATP5A, ATP5AL2, ATPM; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ATP5A1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAFC-1 is a rabbit monoclonal antibody that recognizes ATP synthase F(1) complex subunit alpha, mitochondrial (gene ATP5F1A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 59.8 kDa, and the supplier reports an observed band at about 50 kDa.
ATP5F1A is the α subunit of the F1 catalytic head of mitochondrial ATP synthase (complex V). Three α and three β subunits form the ring in which ATP is made from ADP and phosphate, powered by the proton gradient across the inner mitochondrial membrane.
| Target | ATP synthase F(1) complex subunit alpha, mitochondrial (gene ATP5F1A; UniProt P25705, human) |
|---|---|
| Clone | AAFC-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 59.8 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:20 |
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ATP5A1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP synthase F(1) complex subunit alpha, mitochondrial in the mitochondrion, mitochondrion inner membrane and cell membrane. Tissue expression noted by UniProt (human): Fetal lung, heart, liver, gut and kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
