| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP synthase F; 1; complex subunit alpha, mitochondrial; ATP synthase F1 subunit alpha; ATP5F1A; ATP5A; ATP5A1; ATP5AL2; ATPM |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATP5F1A recombinant protein (Position: R12-Q520). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes ATP synthase F(1) complex subunit alpha, mitochondrial (gene ATP5F1A) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and ELISA. The predicted molecular weight is 59.8 kDa, and the supplier reports an observed band at about 54 kDa.
ATP5F1A is the α subunit of the F1 catalytic head of mitochondrial ATP synthase (complex V). Three α and three β subunits form the ring in which ATP is made from ADP and phosphate, powered by the proton gradient across the inner mitochondrial membrane.
| Target | ATP synthase F(1) complex subunit alpha, mitochondrial (gene ATP5F1A; UniProt P25705, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 59.8 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human SK-OV-3 cells, human MCF-7 cells, human HL-60 cells, human RT4 cells, human Daudi cells, human THP-1 cells, human HepG2 cells, rat heart tissue, rat brain tissue, rat lung tissue, rat H9C2(2–1) cells, mouse heart tissue, mouse brain tissue, mouse lung tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human endometrial adenocarcinoma tissue, human placenta tissue, human rectal cancer tissue, human thyroid cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): HepG2 cells.
Samples with confirmed staining (ICC/IF): human placenta tissue, human colon cancer tissue.
Samples with a confirmed signal (flow cytometry): U937 cells.
Immunogen
Recombinant human ATP5F1A fragment (Arg12–Gln520), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP synthase F(1) complex subunit alpha, mitochondrial in the mitochondrion, mitochondrion inner membrane and cell membrane. Tissue expression noted by UniProt (human): Fetal lung, heart, liver, gut and kidney. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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