| Field | Specification |
|---|---|
| Target | |
| Alternative names | V-type proton ATPase subunit B, kidney isoform; V-ATPase subunit B 1; Endomembrane proton pump 58 kDa subunit; Vacuolar proton pump subunit B 1; ATP6V1B1; ATP6B1; VATB; VPP3 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ATP6V1B1 recombinant protein (Position: C17-L513). Human ATP6V1B1 shares 95.2% and 88.2% amino acid (aa) sequence identity with mouse and rat ATP6V1B1, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets V-type proton ATPase subunit B, kidney isoform (gene ATP6V1B1) in human samples and is validated for Western blot and ELISA.
ATP6V1B1 is the kidney isoform of the B subunit of the vacuolar H+-ATPase (V-ATPase). In kidney intercalated cells it supports acid secretion into urine, and mutations cause distal renal tubular acidosis with sensorineural deafness.
| Target | V-type proton ATPase subunit B, kidney isoform (gene ATP6V1B1; UniProt P15313, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human ATP6V1B1 fragment (Cys17–Leu513), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 95.2% and 88.2%, respectively.
Reactivity Notes
The supplier lists reactivity with human. UniProt places V-type proton ATPase subunit B, kidney isoform in the apical cell membrane and basolateral cell membrane. Tissue expression noted by UniProt (human): Kidney.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
